Quantitative Determination of Catechins by Paper Partition Chromatography

The quantitative separation of estrogens by paper partition chromatography.

The varying success of known chromat’ographic procedures for the separation and identification of microquantities of estrogens (1, 2) has led to the development of paper partit!ion chromatographic techniques which are reported here. These t’echniques permit rapid separation, identification, and quantitative estimation of free est.rogens in biological materials. The solvent systems of o-dichloro...

Determination of monoamino monocarboxylic acids by quantitative paper chromatography.

Many methods for chromatographic separation of amino acids are now available, but their subsequent determination, generally employing ninhydrin, often leaves much to be desired. It was felt, therefore, that there was a need for a simple method of analysis based on the convenient paper technique and employing some means of determination less fickle than ninhydrin colorimetry. The method now pres...

Attomole catechins determination by capillary liquid chromatography with electrochemical detection.

Attomole quantities of catechins were determined by a capillary liquid chromatography system with electrochemical detection (CLC-ECD) and the system is applied to the determination of catechins in human plasma. The eight catechins: catechin (C), epicatechin (EC), gallocatechin (GC), epigallocatechin (EGC), catechin gallate (Cg), epicatechin gallate (ECg), gallocatechin gallate (GCg), and epigal...

Study of phenylketonuria by paper chromatography

DEVELOPMENT OF A METHOD FOR THE DETERMINATION OF 4-HYDROXY-3-METHOXYMANDELIC ACID (VANILLYLMANDELIC ACID) IN URINE BY PAPER CHROMATOGRAPHY

We have developed a simple and precise paper chromatographic method for the determination of 4-hydroxy-3-methoxymandelic acid (VMA) in urine. Concentrations of VMA in patients with neuroblastoma were increased in comparison to controls. The linearity was excellent in the concentration range tested. The within-assay coefficient of variation for control and patient urine was less than 2.2%. ...